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  • KD/KO Validated

ASPM Polyclonal antibody

ASPM Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 26223-1-AP

Synonyms

ASP, Asp homolog, ASPM, Calmbp1, MCPH5



Tested Applications

Positive WB detected inHEK-293T cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue, human gliomas tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26223-1-AP is ASPM Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASPM fusion protein Ag24166
Full Name asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
Calculated molecular weight410 kDa or 218 kDa
Observed molecular weight 218 kDa
GenBank accession numberBC034607
Gene symbol ASPM
Gene ID (NCBI) 259266
RRIDAB_2880431
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ASPM antibody 26223-1-APDownload protocol
IHC protocol for ASPM antibody 26223-1-APDownload protocol
IF protocol for ASPM antibody 26223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IP

Aging (Albany NY)

ASPM promotes glioblastoma growth by regulating G1 restriction point progression and Wnt-β-catenin signaling.

Authors - Xin Chen
  • KD Validated
humanIHC

Int J Cancer

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Authors - Fangqiu Fu
humanWB

Front Oncol

Identifying and Validating Potential Biomarkers of Early Stage Lung Adenocarcinoma Diagnosis and Prognosis.

Authors - Yingji Chen
humanWB

DNA Cell Biol

ASPM is a Novel Candidate Gene Associated with Colorectal Cancer Cell Growth.

Authors - Qian Yang
humanWB,IHC

Int J Endocrinol

ASPM Promotes the Progression of Anaplastic Thyroid Carcinomas by Regulating the Wnt/β-Catenin Signaling Pathway.

Authors - Liang Jiang
  • KD Validated
humanWB,IHC

Gland Surg

Abnormal spindle-like microcephaly-associated protein promotes proliferation by regulating cell cycle in epithelial ovarian cancer.

Authors - Yiguo Wu
  • KD Validated