Validation Data Gallery
|Positive WB detected in||HepG2 cells, human placenta tissue, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
60035-1-Ig targets ATF4 in WB, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ATF4 fusion protein Ag1279|
|Full Name||activating transcription factor 4 (tax-responsive enhancer element B67)|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC022088|
|Gene ID (NCBI)||468|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
What is the molecular weight of ATF4?
The molecular weight of ATF is 38.6 kD.
What is ATF4?
Activating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor (PMIDs: 16000305, 17485283).
What the function of ATF4?
ATF4 its a transcription factor that controls the transcriptional activity of mature osteoblasts. ATF4 is particularly critical for their timely onset and terminal differentiation, as well as expression of Bsp and osteocalcin. Knockout animals displayed reduction or delay in bone mineralization and have severely reduced bone volume. ATF4 is also part of the PERK-eIF2α-ATF4-CHOP apoptosis pathway, which is activated by ER stress, and it likely plays a role related to tumor cell survival (PMIDs: 18083928, 16000305, 30134550).
What is the effect of ATF4 interaction with RSK2?
ATF4 and RSK2 posttranscriptionally regulate type I collagen synthesis. Lack of RSK2 phosphorylation of AFT4 may contribute to skeletal phenotypes associated with Coffin-Lowry Syndrome (PMID: 17485283).
Where is ATF4 expressed?
ATF4 protein is predominantly expressed in osteoblasts, although its corresponding Atf4 mRNA is ubiquitously expressed (PMID: 16000305).
What regulates ATF4 expression?
ATF4 is regulated by a ubiquitin/proteasomal pathway, which is less active in osteoblasts by inhibition with MG115 (PMID: 16000305).
How does ATF4 expression affect Ocn mRNA?
Inhibition of the degradation pathway leads to ATF4 accumulation and induces Ocn mRNA expression in non-osteoblastic cells (PMID: 16000305).
Does ATF4 have the ability to induce osteoblast-specific gene expression even in non-osteoblastic cells?
Yes, ATF4, as well as other osteoblast differentiation factors, has this ability. AFT4 interactions with Runx2 can stimulate osteoblast-specific osteocalcin gene expression. (PMIDs: 16000305, 17485283)
J Clin Invest
ATF4-dependent induction of heme oxygenase 1 prevents anoikis and promotes metastasis.
Reducing ER stress with chaperone therapy reverses sleep fragmentation and cognitive decline in aged mice.
Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.
Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.
The portrait of liver cancer is shaped by mitochondrial genetics.
Cell Mol Life Sci
Sestrin2 protects against lethal sepsis by suppressing the pyroptosis of dendritic cells
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Daniel (Verified Customer) (12-02-2019)
Multiple bands appeared which were higher than the expected ~50kDa.