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CoraLite®594-conjugated ATF4 Monoclonal antibody

ATF4 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E4C5

Cat no : CL594-60035

Synonyms

ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, TAXREB67, TXREB

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated ATF4 Monoclonal antibody (CL594-60035)

    FC experiment of HeLa using CL594-60035

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human ATF4 (CL594-60035, Clone:3E4C5) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

CL594-60035 targets ATF4 in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATF4 fusion protein Ag1279
Full Name activating transcription factor 4 (tax-responsive enhancer element B67)
Calculated molecular weight 39 kDa
GenBank accession numberBC022088
Gene symbol ATF4
Gene ID (NCBI) 468
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. This antibody is a mouse monoclonal antibody raised against full length human ATF4 antigen. This anitbody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
FC protocol for CL594 ATF4 antibody CL594-60035Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Authors - Zhixiong Liu
View Article