ATG9A Monoclonal antibody, PBS Only

ATG9A Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D12E11

Cat no : 67096-1-PBS

Synonyms

APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208

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Validation Data Gallery

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  WB analysis using 67096-1-Ig (same clone as 67096-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67096-1-Ig (ATG9A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 67096-1-Ig (same clone as 67096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67096-1-Ig (ATG9A antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 67096-1-Ig (same clone as 67096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67096-1-Ig (ATG9A antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67096-1-Ig (same clone as 67096-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67096-1-Ig (ATG9A antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67096-1-PBS targets ATG9A in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATG9A fusion protein Ag24278
• Full Name: ATG9 autophagy related 9 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 837 aa, 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC065534
• Gene Symbol: ATG9A
• Gene ID (NCBI): 79065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)