ATP6V1C1 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 86345-2-RR (same clone as 86345-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

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  Sample test of MP02350-1

  The mean ATP6V1C1 concentration was determined to be 29.6 ng/mL in NIH/3T3 cell extract based on a 1.4 mg/mL extract load.

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  Cytometric bead array standard curve of MP02350-1

  Cytometric bead array standard curve of MP02350-1, ATP6V1C1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86345-2-PBS. Detection antibody: 86345-1-PBS. Standard: Ag9020. Range: 1.562-200 ng/mL.

ATP6V1C1 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 86345-2-PBS

Clone No.250958C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

ATP6C, ATP6D, VATC, V-ATPase subunit C 1, Vma5

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Product Information

86345-2-PBS targets ATP6V1C1 as part of a matched antibody pair:

MP02350-1: 86345-2-PBS capture and 86345-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATP6V1C1 fusion protein Ag9020
• Full Name: ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
• Calculated Molecular Weight: 382 aa, 44 kDa
• Observed Molecular Weight: 42-44 kDa
• GenBank Accession Number: BC010960
• Gene Symbol: ATP6V1C1
• Gene ID (NCBI): 528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21283
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The vacuolar H+-ATPase (V-ATPase) is a functionally conserved multimeric complex localized at the membranes of many organelles where its proton-pumping action is required for proper lumen acidification (PMID: 39210597). ATP6V1C1, also known as ATPase, H+ transporting, lysosomal V1 subunit C1, is a component of the V1 sector of the V-ATPase complex. ATP6v1c1 knockdown significantly reduces tumor stimulated bone resorption through osteoclastogenesis at the bone and metastasis in vivo, as well as V-ATPase activity, proliferation, and mTORC1 activation in vitro (PMID: 28504970). Also, ATP6V1C1, associated with the tumor microenvironment and mTORC1 signaling pathway, is a potential diagnostic, prognostic, and therapeutic biomarker for hepatocellular carcinoma () (PMID: 39557733).