ATP6V1C1 Recombinant antibody

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  WB analysis using 86345-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ATP6V1C1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86345-2-RR

Clone No.250958C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ATP6C, ATP6D, VATC, V-ATPase subunit C 1, Vma5

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Tested Applications

• Positive WB detected in: HeLa cells, U-251 cells, NIH/3T3 cells, mouse brain tissue, fetal human brain tissue, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86345-2-RR targets ATP6V1C1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATP6V1C1 fusion protein Ag9020
• Full Name: ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
• Calculated Molecular Weight: 382 aa, 44 kDa
• Observed Molecular Weight: 42-44 kDa
• GenBank Accession Number: BC010960
• Gene Symbol: ATP6V1C1
• Gene ID (NCBI): 528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21283
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The vacuolar H+-ATPase (V-ATPase) is a functionally conserved multimeric complex localized at the membranes of many organelles where its proton-pumping action is required for proper lumen acidification (PMID: 39210597). ATP6V1C1, also known as ATPase, H+ transporting, lysosomal V1 subunit C1, is a component of the V1 sector of the V-ATPase complex. ATP6v1c1 knockdown significantly reduces tumor stimulated bone resorption through osteoclastogenesis at the bone and metastasis in vivo, as well as V-ATPase activity, proliferation, and mTORC1 activation in vitro (PMID: 28504970). Also, ATP6V1C1, associated with the tumor microenvironment and mTORC1 signaling pathway, is a potential diagnostic, prognostic, and therapeutic biomarker for hepatocellular carcinoma () (PMID: 39557733).