Aggrecan Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 84819-5-RR (same clone as 84819-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84819-5-RR (Aggrecan antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84819-5-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01577-3

  The mean Aggrecan concentration was determined to be 12.09 ng/mL in U2OS cell extract based on a 1.30 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01577-3

  Sandwich ELISA standard curve of MP01577-3, Human Aggrecan Recombinant Matched Antibody Pair - PBS only. 84819-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2994. 84819-6-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

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  IHC staining of mouse lung using 84819-5-RR (same clone as 84819-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84819-5-RR (Aggrecan antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84819-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 84819-5-RR (same clone as 84819-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84819-5-RR (Aggrecan antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84819-5-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 84819-5-RR (same clone as 84819-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84819-5-RR (Aggrecan antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84819-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 84819-5-RR (same clone as 84819-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84819-5-RR (Aggrecan antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84819-5-PBS in a different storage buffer formulation.

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  Affinity of 84819-5-RR

  Biolayer interferometry (BLl) kinetic assays of 88419-5-RR against Human Aggrecan were performed. The affinity constant is 0.124 nM.

Aggrecan Uni-rAb^® Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Cat No. 84819-5-PBS

Clone No.250777C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Synonyms

ACAN, AGC1, AGCAN, Aggrecan 1, Aggrecan core protein 2

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Product Information

84819-5-PBS targets Aggrecan as part of a matched antibody pair:

MP01577-3: 84819-5-PBS capture and 84819-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2994

  Product name: Recombinant Human ACAN protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 20-675 aa of NM_013227.4

  Sequence: VETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFR

• Full Name: aggrecan
• Calculated Molecular Weight: 261 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: NM_013227.4
• Gene Symbol: ACAN
• Gene ID (NCBI): 176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16112-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.