Filter: AllWBIHCAffinity

WB analysis using 84819-5-RR
Various lysates were subjected to SDS PAGE followed by western blot with 84819-5-RR (Aggrecan antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse lung using 84819-5-RR
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84819-5-RR (Aggrecan antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 84819-5-RR
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84819-5-RR (Aggrecan antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 84819-5-RR
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84819-5-RR (Aggrecan antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 84819-5-RR
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84819-5-RR (Aggrecan antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Affinity of 84819-5-RR
Biolayer interferometry (BLl) kinetic assays of 88419-5-RR against Human Aggrecan were performed. The affinity constant is 0.124 nM.

Aggrecan Recombinant monoclonal antibody

Name: Aggrecan Recombinant monoclonal antibody
Brand: Proteintech
SKU: 84819-5-RR

Aggrecan Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84819-5-RR

Clone No.250777C8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ACAN, AGC1, AGCAN, Aggrecan 1, Aggrecan core protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse cartilage tissue, rat auricular cartilage tissue
Positive IHC detected in	human lung cancer tissue, mouse lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84819-5-RR targets Aggrecan in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2994 Product name: Recombinant Human ACAN protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 20-675 aa of NM_013227.4 Sequence: VETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFR Predict reactive species
Full Name	aggrecan
Calculated Molecular Weight	261 kDa
Observed Molecular Weight	140-150 kDa
GenBank Accession Number	NM_013227.4
Gene Symbol	ACAN
Gene ID (NCBI)	176
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P16112-1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.