Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 66121-1-Ig (same clone as 66121-1-PBS)
WB result of GLA antibody (66121-1-Ig, 1:2000) with si-Control and si-GLA transfected HEK293 cells.. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
WB analysis using 66121-1-Ig (same clone as 66121-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
WB analysis of LNCaP using 66121-1-Ig (same clone as 66121-1-PBS)
LNCaP cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66121-1-Ig (same clone as 66121-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 66121-1-Ig (same clone as 66121-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66121-1-Ig (same clone as 66121-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
WB analysis of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66121-1-Ig (same clone as 66121-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66121-1-Ig (same clone as 66121-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66121-1-Ig (same clone as 66121-1-PBS)
Immunofluorescent analysis of HepG2 cells using 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66121-1-Ig (same clone as 66121-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha Galactosidase A antibody (66121-1-Ig, Clone: 2B2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Alpha Galactosidase A Monoclonal antibody, PBS Only

Alpha Galactosidase A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66121-1-PBS

Clone No.2B2C5

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GLA, 2B2C5, Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase, Alpha-galactosidase A

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66121-1-PBS targets Alpha Galactosidase A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag7505 Product name: Recombinant human GLA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 81-425 aa of BC002689 Sequence: WKDAGYEYLCIDDCWMAPQRDSEGRLQADPQRFPHGIRQLANYVHSKGLKLGIYADVGNKTCAGFPGSFGYYDIDAQTFADWGVDLLKFDGCYCDSLENLADGYKHMSLALNRTGRSIVYSCEWPLYMWPFQKPNYTEIRQYCNHWRNFADIDDSWKSIKSILDWTSFNQERIVDVAGPGGWNDPDMLVIGNFGLSWNQQVTQMALWAIMAAPLFMSNDLRHISPQAKALLQDKDVIAINQDPLGKQGYQLRQGDNFEVWERPLSGLAWAVAMINRQEIGGPRSYTIAVASLGKGVACNPACFITQLLPVKRKLGFYEWTSRLRSHINPTGTVLLQLENTMQMSL Predict reactive species
Full Name	galactosidase, alpha
Calculated Molecular Weight	49 kDa
Observed Molecular Weight	49 kDa
GenBank Accession Number	BC002689
Gene Symbol	GLA
Gene ID (NCBI)	2717
RRID	AB_2881520
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P06280
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

GLA, also named as Melibiase and Alpha-galactosidase A, belongs to the glycosyl hydrolase 27 family. It hydrolyzes terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.