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Alpha Galactosidase A Monoclonal antibody

Alpha Galactosidase A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B2C5

Cat no : 66121-1-Ig

Synonyms

Alpha D galactosidase A, Alpha galactosidase A, galactosidase, alpha



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, human kidney tissue, LNCaP cells, A431 cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66121-1-Ig targets Alpha Galactosidase A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Alpha Galactosidase A fusion protein Ag7505
Full Name galactosidase, alpha
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC002689
Gene Symbol GLA
Gene ID (NCBI) 2717
RRIDAB_2881520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLA, also named as Melibiase and Alpha-galactosidase A, belongs to the glycosyl hydrolase 27 family. It hydrolyzes terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.

Protocols

Product Specific Protocols
WB protocol for Alpha Galactosidase A antibody 66121-1-IgDownload protocol
IHC protocol for Alpha Galactosidase A antibody 66121-1-IgDownload protocol
IF protocol for Alpha Galactosidase A antibody 66121-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

bioRxiv

Antigen-display exosomes provide adjuvant-free protection against SARS-CoV-2 disease at nanogram levels of spike protein

Authors - Chenxu Guo
humanWB

Nat Commun

Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis

Authors - Sebastian Boland