Angiogenin Recombinant antibody

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  WB analysis using 85635-1-RR

  Untreated and Brefeldin A treated HepG2 cells were subjected to SDS PAGE followed by western blot with 85635-1-RR (ANG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85635-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ANG antibody (85635-1-RR, Clone: 242738D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Angiogenin Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85635-1-RR

Clone No.242738D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ANG, ANG 1, ANG I, ANG1, ANGI

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Tested Applications

• Positive WB detected in: Brefeldin A treated HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85635-1-RR targets Angiogenin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Angiogenin fusion protein Eg1930
• Full Name: angiogenin, ribonuclease, RNase A family, 5
• Calculated Molecular Weight: 147 aa, 17 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC054880
• Gene Symbol: Angiogenin
• Gene ID (NCBI): 283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P03950
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC. It is a 17 kDa precursor which is cleaved to generate the 14 kDa mature protein.