AuthentiKine™ Human IFN alpha 2A ELISA Kit
Cat no : KE00207
Synonyms
IFN alpha 2, IFNA2, IFN alpha 2A, IFN alpha2, IFN-alpha 2
Validation Data Gallery
Product Information
KE00207 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN alpha 2A ELISA kit is to be used to detect and quantify protein levels of endogenous IFN alpha 2A. The assay recognizes human IFN alpha 2A. An antibody specific for IFN alpha 2A has been pre-coated onto the microwells. The IFN alpha 2A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IFN alpha 2A is added to detect the captured IFN alpha 2A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IFN alpha 2A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3440 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 96% | 85%-104% |
| Human Plasma | 98% | 82%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 238.7 | 3.5 | 1.4 |
| 2 | 20 | 55.2 | 0.8 | 1.5 |
| 3 | 20 | 13.1 | 0.6 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 231.1 | 4.4 | 1.9 |
| 2 | 24 | 53.8 | 1.0 | 1.9 |
| 3 | 24 | 13.7 | 0.7 | 5.2 |
Background Information
Human interferon alpha-2 (IFNA2), is a member of the Type I interferon cytokine family, known for its antiviral and anti-proliferative functions. Several IFN alpha2 alleles have been described, and the best known are alpha-2a and alpha-2b. IFN alpha2 binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. interferon alpha 2 is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. Interferon alpha-2 products, such as interferon alpha-2a (IFNA2) are used to treat multiple diseases such as hairy cell leukemia and hepatitis c.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IFN alpha 2, IFNA2, IFN alpha 2A, IFN alpha2, IFN-alpha 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Rep IFNα-induced BST2+ tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling |