AuthentiKine™ Human CXCL8/IL-8 ELISA Kit
Cat no : KE00275
Synonyms
CXCL8,IL8,IL-8,C X C motif chemokine 8,Chemokine
Validation Data Gallery
Product Information
KE00275 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CXCL8/IL-8 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL8/IL-8. The assay recognizes human CXCL8/IL-8. An antibody specific for CXCL8/IL-8 has been pre-coated onto the microwells. The CXCL8/IL-8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human CXCL8/IL-8 is added to detect the captured human CXCL8/IL-8 protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human CXCL8/IL-8 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 1.56-100 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3576 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 107% | 76%-128% |
| Cell culture supernatants | 106% | 86%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 86.5 | 3.8 | 4.4 |
| 2 | 20 | 19.9 | 0.3 | 1.6 |
| 3 | 20 | 5.0 | 0.1 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 82.0 | 4.2 | 5.1 |
| 2 | 24 | 19.3 | 0.7 | 3.4 |
| 3 | 24 | 4.5 | 0.3 | 7.4 |
Background Information
Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CXCL8,IL8,IL-8,C X C motif chemokine 8,Chemokine |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Phytomedicine Tetrandrine alleviates macrophage activation syndrome after CAR-T cell therapy | ||
Heliyon Abnormal expression of CEBPB promotes the progression of renal cell carcinoma through regulating the generation of IL-6 | ||
Int J Biol Sci Targeting the LHX1-LDB1 Complex Restores STING-dependent Senescence Surveillance and Inhibits Head and Neck Cancer Progression. | ||
Adv Sci (Weinh) Exosomes-transferred LINC00668 Contributes to Thrombosis by Promoting NETs Formation in Inflammatory Bowel Disease | ||
Sci Rep Protective effects of a new generation of probiotic Bacteroides fragilis against colitis in vitro and in vitro | ||
J Cancer Matrix stiffness regulates NPC invasiveness by modulating a mechanoresponsive TRPV4-Nox4-IL-8 signaling axis |