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- KD/KO Validated
BAX Monoclonal antibody
BAX Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
human, mouse, rat and More (2)
WB, IHC, IF, ICC, FC, CoIP, ELISA
Cat no : 60267-1-Ig
|Positive WB detected in||HeLa cells, human testis tissue, HEK-293 cells, COLO 320 cells, PC-12 cells, ROS1728 cells, Neuro-2a cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 267 publications below|
|IHC||See 18 publications below|
|IF||See 15 publications below|
|CoIP||See 1 publications below|
60267-1-Ig targets BAX in WB, IHC, IF, ICC, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, canine, pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||BAX fusion protein Ag21068|
|Full Name||BCL2-associated X protein|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC014175|
|Gene ID (NCBI)||581|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).
|Product Specific Protocols|
|WB protocol for BAX antibody 60267-1-Ig||Download protocol|
|IHC protocol for BAX antibody 60267-1-Ig||Download protocol|
|FC protocol for BAX antibody 60267-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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