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BAX Antibody 18 Publications

Mouse Monoclonal| Catalog number: 60267-1-Ig |CloneNo.: 4G5E8

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Con: 240 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

BAX Antibody WB MCF-7 cells 60267-1-Ig

MCF-7 cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig( BAX Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

BAX Antibody WB HEK-293 cells 60267-1-Ig

HEK-293, ROS1728, C6, PC-12, Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig( BAX Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

BAX Antibody IHC human breast cancer tissue 60267-1-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60267-1-Ig( BAX Antibody) at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

BAX Antibody IHC human breast cancer tissue 60267-1-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60267-1-Ig( BAX Antibody) at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

BAX Antibody IF MCF-7 cells 60267-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed MCF-7 cells using 60267-1-Ig(BAX antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
MCF-7 cells, human testis tissue, HEK-293 cells, HepG2 cells, HeLa cells
Positive IHC detected in:
human breast cancer tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells, HeLa cells
Recommended dilution:
WB : 1:1000-1:10000
IHC : 1:400-1:1000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
BAX fusion protein Ag21068
Full name:
BCL2-associated X protein
Calculated molecular weight:
21 kDa
Observed molecular weight:
21 kDa
GenBank accession number:
BC014175
Gene ID (NCBI):
581
Gene symbol
BAX
Synonyms
Apoptosis regulator BAX, BAX

Background

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).


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