BAX Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

BAX Monoclonal Antibody for WB, IP, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IP, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G5E8

Cat no : 60267-1-PBS

Synonyms

Apoptosis regulator BAX, BAX

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Validation Data Gallery

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  WB analysis using 60267-1-Ig (same clone as 60267-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 60267-1-Ig (same clone as 60267-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60267-1-Ig (same clone as 60267-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  IP experiment of THP-1 using 60267-1-Ig (same clone as 60267-1-PBS)

  IP result of anti-BAX (IP:60267-1-Ig, 4ug; Detection:60267-1-Ig 1:6000) with THP-1 cells lysate 1520 ug. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 60267-1-Ig (same clone as 60267-1-PBS)

  1X10^6 MCF-7 cells were stained with 0.2 ug Anti-Human BAX (60267-1-Ig, Clone:4G5E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60267-1-PBS targets BAX in WB, IP, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: BAX fusion protein Ag21068
• Full Name: BCL2-associated X protein
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC014175
• Gene Symbol: BAX
• Gene ID (NCBI): 581
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).