CoraLite®555-conjugated BAX Monoclonal antibody
BAX Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
4G5E8
Cat no : CL555-60267
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | Ramos cells |
Recommended dilution
Application | Dilution |
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Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL555-60267 is BAX Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | BAX fusion protein Ag21068 |
Full Name | BCL2-associated X protein |
Calculated molecular weight | 21 kDa |
GenBank accession number | BC014175 |
Gene symbol | BAX |
Gene ID (NCBI) | 581 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 557 nm / 570nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).
Protocols
Product Specific Protocols | |
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FC protocol for CL555 BAX antibody CL555-60267 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |