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CoraLite®555-conjugated BAX Monoclonal antibody

BAX Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4G5E8

Cat no : CL555-60267

Synonyms

Apoptosis regulator BAX, BAX

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Ramos cells using CoraLite®555-conjugated BAX Monoclonal antibody (CL555-60267)

    FC experiment of Ramos using CL555-60267

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human BAX (CL555-60267, Clone:4G5E8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inRamos cells

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL555-60267 is BAX Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BAX fusion protein Ag21068
Full Name BCL2-associated X protein
Calculated molecular weight 21 kDa
GenBank accession numberBC014175
Gene symbol BAX
Gene ID (NCBI) 581
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).

Protocols

Product Specific Protocols
FC protocol for CL555 BAX antibody CL555-60267Download protocol
Standard Protocols
Click here to view our Standard Protocols