BDKRB1 Polyclonal antibody

BDKRB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26672-1-AP

Synonyms

B1 bradykinin receptor, B1BKR, B1R, BDKRB1, BK 1 receptor, BKB1R, BKR1, BRADYB1, bradykinin receptor B1

---

Validation Data Gallery

• 

  WB analysis of HUVEC using 26672-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 26672-1-AP (BDKRB1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 26672-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26672-1-AP (BDKRB1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26672-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26672-1-AP (BDKRB1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 26672-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26672-1-AP (BDKRB1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 26672-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26672-1-AP (BDKRB1 Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HUVEC cells
• Positive IHC detected in: human kidney tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 26672-1-AP is BDKRB1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BDKRB1 fusion protein Ag24745
• Full Name: bradykinin receptor B1
• Calculated Molecular Weight: 353 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC034705
• Gene Symbol: BDKRB1
• Gene ID (NCBI): 623
• RRID: AB_2880596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Front Endocrinol (Lausanne); The Bradykinin System Contributes to the Regulation osungangrenji@hotmail.comf Prostaglandin-Endoperoxide Synthase 2 Expression in Human Amnion Fibroblasts: Implications for Term and Preterm Birth.; Authors - Xiao-Tian Ni
human	WB	Pharmaceuticals (Basel); Assessment of Radiolabelled Derivatives of R954 for Detection of Bradykinin B1 Receptor in Cancer Cells: Studies on Glioblastoma Xenografts in Mice; Authors - Miho Shukuri