Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | mouse ovary tissue, human medulloblastoma tissue, human placenta tissue, rat brain tissue, human ovary cancer tissue, human appendicitis tissue, rat ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12390-1-AP targets BEX1/2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3066 Product name: Recombinant human BEX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC015522 Sequence: MESKEERALNNLIVENVNQENDEKDEKEQVANKGEPLALPLNVSEYCVPRGNRRRFRVRQPILQYRWDIMHRLGEPQARMREENMERIGEEVRQLMEKLREKQLSHSLRAVSTDPPHHDHHDEFCLMP Predict reactive species |
| Full Name | brain expressed X-linked 2 |
| Calculated Molecular Weight | 128 aa, 15 kDa |
| Observed Molecular Weight | 10-15 kDa |
| GenBank Accession Number | BC015522 |
| Gene Symbol | BEX2 |
| Gene ID (NCBI) | 84707 |
| RRID | AB_10644326 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BEX1 is a signaling adapter molecule involved in p75NTR/NGFR signaling. It lays a role in cell cycle progression and neuronal differentiation. BEX1 inhibits neuronal differentiation in response to nerve growth factor (NGF). BEX2 is a regulator of mitochondrial apoptosis and G1 cell cycle in breast cancer. It regulates the level of PP2A regulatory subunit B and PP2A phosphatase activity. The homology between human BEX1 and BEX2 is 94%. This antibody detects both BEX1 and BEX2.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BEX1/2 antibody 12390-1-AP | Download protocol |
| WB protocol for BEX1/2 antibody 12390-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BEX1/2 Polyclonal antibody (Cat# 12390-1-AP) has 6 citations published in Cell Death & Disease, International Journal of Biological Sciences, BMC Biology, Frontiers in Cell and Developmental Biology, Translational Oncology, and Aging. Associated research fields include mitophagy and apoptosis in lung cancer, tumor proliferation in colorectal cancer, ciliogenesis, cuproptosis in hepatocellular carcinoma, immunotherapy in renal cell carcinoma, and ferroptosis in osteoarthritis.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Crotonylated BEX2 interacts with NDP52 and enhances mitophagy to modulate chemotherapeutic agent-induced apoptosis in non-small-cell lung cancer cells | ||
BMC Biol Bex1 is essential for ciliogenesis and harbours biomolecular condensate-forming capacity. | ||
Front Cell Dev Biol Single-cell and genetic multi-omics analysis combined with experiments confirmed the signature and potential targets of cuproptosis in hepatocellular carcinoma | ||
Transl Oncol LMO2 confers value as a potential immunotherapy marker in pan-cancer analysis and inhibits progression of Clear Cell Renal Cell Carcinoma | ||
Aging (Albany NY) Machine learning-based identification and immune characterization of ferroptosis-related molecular clusters in osteoarthritis and validation |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The user reported that the antibody performed well for Western Blot on mouse liver tissue when diluted at 1:1000 in 1X TBST. Overall, the feedback confirms reliable performance for its intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kamal (Verified Customer) (02-15-2024) | This antibody works for mouse liver tissue when diluted in 1X TBST.
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