BEX1/2 Polyclonal antibody

BEX1/2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 12390-1-AP

Synonyms

BEX1, BEX1/2, BEX2, brain expressed X linked 2, hBex2, Protein BEX2

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Validation Data Gallery

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  WB analysis of human brain using 12390-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12390-1-AP (BEX1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human medulloblastoma using 12390-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12390-1-AP (BEX1/2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human medulloblastoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12390-1-AP targets BEX1/2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BEX1/2 fusion protein Ag3066
• Full Name: brain expressed X-linked 2
• Calculated Molecular Weight: 128 aa, 15 kDa
• Observed Molecular Weight: 10-15 kDa
• GenBank Accession Number: BC015522
• Gene Symbol: BEX2
• Gene ID (NCBI): 84707
• RRID: AB_10644326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BEX1 is a signaling adapter molecule involved in p75NTR/NGFR signaling. It lays a role in cell cycle progression and neuronal differentiation. BEX1 inhibits neuronal differentiation in response to nerve growth factor (NGF). BEX2 is a regulator of mitochondrial apoptosis and G1 cell cycle in breast cancer. It regulates the level of PP2A regulatory subunit B and PP2A phosphatase activity. The homology between human BEX1 and BEX2 is 94%. This antibody detects both BEX1 and BEX2.

Publications

Species	Application	Title
human,mouse	IF	BMC Biol; Bex1 is essential for ciliogenesis and harbours biomolecular condensate-forming capacity.; Authors - Emi Hibino
human	WB	Int J Biol Sci; BEX2 promotes tumor proliferation in colorectal cancer.; Authors - Yeting Hu
human	WB	Front Cell Dev Biol; Single-cell and genetic multi-omics analysis combined with experiments confirmed the signature and potential targets of cuproptosis in hepatocellular carcinoma; Authors - Feng Cao
human,mouse	IHC,IF	Cell Death Dis; Crotonylated BEX2 interacts with NDP52 and enhances mitophagy to modulate chemotherapeutic agent-induced apoptosis in non-small-cell lung cancer cells; Authors - Ning Mu
human	WB	Aging (Albany NY); Machine learning-based identification and immune characterization of ferroptosis-related molecular clusters in osteoarthritis and validation; Authors - Xiaocheng Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

This antibody works for mouse liver tissue when diluted in 1X TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver tissue