• Featured Product
  • KD/KO Validated

TGFBI / BIGH3 Polyclonal antibody

TGFBI / BIGH3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)


WB, Neutralization, IP, IHC, IF, FC, ELISA



Cat no : 10188-1-AP


Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3

Tested Applications

Positive WB detected inmouse eye tissue, mouse liver tissue, human kidney tissue, Y79 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue, mouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inTGF beta 1 treated A549 cells
Positive FC detected inY79 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10188-1-AP targets TGFBI / BIGH3 in WB, Neutralization, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGFBI / BIGH3 fusion protein Ag0241
Full Name transforming growth factor, beta-induced, 68kDa
Calculated molecular weight 683 aa, 75 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC000097
Gene symbol TGFBI
Gene ID (NCBI) 7045
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).


Product Specific Protocols
WB protocol for TGFBI / BIGH3 antibody 10188-1-APDownload protocol
IHC protocol for TGFBI / BIGH3 antibody 10188-1-APDownload protocol
IF protocol for TGFBI / BIGH3 antibody 10188-1-APDownload protocol
IP protocol for TGFBI / BIGH3 antibody 10188-1-APDownload protocol
FC protocol for TGFBI / BIGH3 antibody 10188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


humanWB, IF

Nat Cell Biol

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets.

Authors - Qin Zhang

Nat Protoc

Comprehensive isolation of extracellular vesicles and nanoparticles

Authors - Qin Zhang

Sci Adv

Acetylated K676 TGFBIp as a severity diagnostic blood biomarker for SARS-CoV-2 pneumonia.

Authors - Hee Ho Park

Genes Dev

Extracellular matrix protein betaig-h3/TGFBI promotes metastasis of colon cancer by enhancing cell extravasation.

Authors - Ma Chaoyu C
  • KD Validated


Dual peptide-dendrimer conjugate inhibits acetylation of transforming growth factor β-induced protein and improves survival in sepsis.

Authors - Wonhwa Lee


TGFBI secreted by tumor-associated macrophages promotes glioblastoma stem cell-driven tumor growth via integrin αvβ5-Src-Stat3 signaling.

Authors - Peng Peng


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Carmelo (Verified Customer) (04-25-2022)

I tested the abs 1:1000 ON at 4C on 40ug spinal cord protein lysates. unfortunately I was not able to distinguish a proper band at the expected size. I will run further optimisation in the incoming weeks.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Spinal Cord
TGFBI / BIGH3 Antibody Western Blot validation (1:1000 dilution) in Mouse Spinal Cord (Cat no:10188-1-AP)