BRD8 Recombinant antibody

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  WB analysis using 84965-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84965-1-RR (BRD8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84965-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRD8 antibody (84965-1-RR, Clone: 242610F5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

BRD8 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84965-1-RR

Clone No.242610F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

SMAP2, SMAP, p120, Bromodomain-containing protein 8, bromodomain containing 8

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Tested Applications

• Positive WB detected in: U2OS cells, HeLa cells, HEK-293T cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84965-1-RR targets BRD8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BRD8 fusion protein Ag0790
• Full Name: bromodomain containing 8
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 120-170 kDa
• GenBank Accession Number: BC008039
• Gene Symbol: BRD8
• Gene ID (NCBI): 10902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H0E9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine-binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. Interestingly, in the breast cancer cells we detected the 170 kDa endogenous BRD8 protein, which was larger than its calculated 135 kDa molecular weight (MW) from its a.a. residues. The study found that with an anti‐HA tag antibody, we validated the expression of HA‐tagged full‐length BRD8 and four different BRD8 fragments expressed in HEK293T cells. The apparent MWs of these BRD8 full‐length and fragment proteins were also larger than their calculated MWs based on their a.a. residues. (PMID: 37680145)