CoraLite® Plus 488-conjugated BRD8 Recombinant antibody

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  IF Staining of HepG2 using CL488-84965

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 BRD8 antibody (CL488-84965, Clone: 242610F5 ) at dilution of 1:200, CL594-Phalloidin (red).

BRD8 Recombinant Antibody for IF/ICC

Cat No. CL488-84965

Clone No.242610F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

242610F5, bromodomain containing 8, Bromodomain-containing protein 8, p120, SMAP

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84965 targets BRD8 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BRD8 fusion protein Ag0790
• Full Name: bromodomain containing 8
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 120-170 kDa
• GenBank Accession Number: BC008039
• Gene Symbol: BRD8
• Gene ID (NCBI): 10902
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H0E9
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine-binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. Interestingly, in the breast cancer cells we detected the 170 kDa endogenous BRD8 protein, which was larger than its calculated 135 kDa molecular weight (MW) from its a.a. residues. The study found that with an anti‐HA tag antibody, we validated the expression of HA‐tagged full‐length BRD8 and four different BRD8 fragments expressed in HEK293T cells. The apparent MWs of these BRD8 full‐length and fragment proteins were also larger than their calculated MWs based on their a.a. residues. (PMID: 37680145)