Tested Applications
| Positive WB detected in | NIH/3T3 cells, Caco-2 cells, RAW 264.7 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human testis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | 200 Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26967-1-AP targets BCL2L1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25604 Product name: Recombinant human Bcl-xL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC019307 Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVN Predict reactive species |
| Full Name | BCL2-like 1 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC019307 |
| Gene Symbol | BCL2L1 |
| Gene ID (NCBI) | 598 |
| RRID | AB_2880702 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q07817 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for BCL2L1 antibody 26967-1-AP | Download protocol |
| IF protocol for BCL2L1 antibody 26967-1-AP | Download protocol |
| IHC protocol for BCL2L1 antibody 26967-1-AP | Download protocol |
| IP protocol for BCL2L1 antibody 26967-1-AP | Download protocol |
| WB protocol for BCL2L1 antibody 26967-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BCL2L1 (Bcl-xL) polyclonal antibody (Cat# 26967-1-AP) has 87 citations across journals including Molecular Cancer, EMBO Journal, Cell Reports, Phytomedicine, British Journal of Pharmacology, Clinical Cancer Research, Cancer Letters, Frontiers in Pharmacology, and Biochemical Pharmacology. Research fields span apoptosis/cell death, oncology (ovarian, gastric, lung, breast, leukemia), neurology, inflammation, cardiology, liver disease, and mitochondrial biology.
| Species | Application | Title |
|---|---|---|
Mol Cancer Role of the lncRNA ABHD11-AS1 in the tumorigenesis and progression of epithelial ovarian cancer through targeted regulation of RhoC. | ||
Exp Mol Med PBK drives PARP inhibitor resistance through the TRIM37/NFκB axis in ovarian cancer | ||
Redox Biol MPST deficiency promotes intestinal epithelial cell apoptosis and aggravates inflammatory bowel disease via AKT | ||
Microbiome The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids. | ||
J Exp Clin Cancer Res 17β-estradiol upregulates IL6 expression through the ERβ pathway to promote lung adenocarcinoma progression. | ||
Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy |
