Bcl-XL Polyclonal antibody

Bcl-XL Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

68

Cat no : 26967-1-AP

Synonyms

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 26967-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26967-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 26967-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26967-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 26967-1-AP

  IP result of anti-Bcl-XL (IP:26967-1-AP, 4ug; Detection:26967-1-AP 1:1000) with NIH/3T3 cells lysate 2000 ug.

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  IHC staining of human testis using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26967-1-AP (Bcl-XL/S antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 26967-1-AP

  1x10^6 200 Jurkat cells were intracellularly stained with 0.4 ug Bcl-XL Polyclonal antibody (26967-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, RAW 264.7 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human testis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: 200 Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 67 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Product Information

26967-1-AP targets Bcl-XL in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Bcl-XL fusion protein Ag25604
• Full Name: BCL2-like 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC019307
• Gene Symbol: Bcl-XL
• Gene ID (NCBI): 598
• RRID: AB_2880702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy; Authors - Lihao Jin
rat	WB	Microbiome; The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids.; Authors - Weiping Xiao
human	WB	Clin Cancer Res; Long noncoding RNA LINC01234 functions as a competing endogenous RNA to regulate CBFB expression by sponging miR-204-5p in gastric cancer.; Authors - Xin Chen
human	WB	Clin Cancer Res; Inhibition of Bcl-2 Synergistically Enhances the Antileukemic Activity of Midostaurin and Gilteritinib in Preclinical Models of FLT3-mutated Acute Myeloid Leukemia.; Authors - Jun Ma
human	WB	Cell Rep; Developmental potency of human ES cell-derived mesenchymal stem cells revealed in mouse embryos following blastocyst injection; Authors - Borong Huang
mouse	WB	Int J Biol Macromol; CircDYM attenuates microglial apoptosis via CEBPB/ZC3H4 axis in LPS-induced mouse model of depression; Authors - Zhongqiu Zhou