Mouse Beta-2-Microglobulin Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85871-4-RR (same clone as 85871-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85871-4-RR (Beta-2-Microglobulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85871-4-PBS in a different storage buffer formulation.

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  Affinity of 85871-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85871-4-RR against Mouse Beta-2-Microglobulin were performed. The affinity constant is 18.9 nM.

Beta-2-Microglobulin Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85871-4-PBS

Clone No.250172E4

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, Indirect ELISA

Synonyms

B2m, beta 2 microglobulin

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Product Information

85871-4-PBS targets Beta-2-Microglobulin in WB, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3086

  Product name: Recombinant Mouse Beta-2-Microglobulin protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 21-119 aa of NM_009735.3

  Sequence: IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM

• Full Name: beta-2 microglobulin
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: NM_009735.3
• Gene Symbol: B2m
• Gene ID (NCBI): 12010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01887
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.