Beta-2-Microglobulin Recombinant monoclonal antibody

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• Beta-2-Microglobulin Recombinant monoclonal antibody (85871-4-RR)

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  WB analysis using 85871-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85871-4-RR (Beta-2-Microglobulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity of 85871-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85871-4-RR against Mouse Beta-2-Microglobulin were performed. The affinity constant is 18.9 nM.

Beta-2-Microglobulin Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85871-4-RR

Clone No.250172E4

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

B2m, beta 2 microglobulin

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Tested Applications

• Positive WB detected in: J774A.1 cells, RAW 264.7 cells, mouse lung tissue, mouse spleen tissue, rat lung tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85871-4-RR targets Beta-2-Microglobulin in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3086

  Product name: Recombinant Mouse Beta-2-Microglobulin protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 21-119 aa of NM_009735.3

  Sequence: IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM

• Full Name: beta-2 microglobulin
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: NM_009735.3
• Gene Symbol: B2m
• Gene ID (NCBI): 12010
• RRID: AB_3744435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01887
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.