Biglycan Monoclonal antibody

Biglycan Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Mouse

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1E3F2

Cat no : 67275-1-Ig

Synonyms

BGN, biglycan, Bone/cartilage proteoglycan I, DSPG1, PG S1, PGI, SLRR1A

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Validation Data Gallery

• 

  WB analysis of pig skin using 67275-1-Ig

  pig skin tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 67275-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67275-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig colon using 67275-1-Ig

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: pig skin tissue, HepG2 cells, pig colon tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

Sample-dependent, check data in validation data gallery

Product Information

67275-1-Ig targets Biglycan in WB,ELISA applications and shows reactivity with Human, Pig, Mouse samples.

• Tested Reactivity: Human, Pig, Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Biglycan fusion protein Ag8012
• Full Name: biglycan
• Calculated molecular weight: 42 kDa
• Observed molecular weight: 48 kDa
• GenBank accession number: BC004244
• Gene symbol: BGN
• Gene ID (NCBI): 633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).