Biglycan Monoclonal antibody

Biglycan Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Mouse and More (1)

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3F2

Cat no : 67275-1-Ig

Synonyms

BGN, biglycan, Bone/cartilage proteoglycan I, DSPG1, PG S1, PGI, SLRR1A



Tested Applications

Positive WB detected inpig skin tissue, HepG2 cells, pig colon tissue, mouse testis tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue, human lung tissue, human lung cancer tissue, human colon cancer tissue, human stomach cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67275-1-Ig targets Biglycan in WB, FC, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse samples.

Tested Reactivity Human, Pig, Mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Biglycan fusion protein Ag8012
Full Name biglycan
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC004244
Gene Symbol BGN
Gene ID (NCBI) 633
RRIDAB_2882544
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

Protocols

Product Specific Protocols
WB protocol for Biglycan antibody 67275-1-IgDownload protocol
IHC protocol for Biglycan antibody 67275-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Biglycan regulated colorectal cancer progress by modulating enteric neuron-derived IL-10 and abundance of Bacteroides thetaiotaomicron

Authors - Yuyu Xu