Biglycan Monoclonal antibody
Biglycan Monoclonal Antibody for WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Pig, Mouse
Applications
WB,ELISA
Conjugate
Unconjugated
CloneNo.
1E3F2
Cat no : 67275-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig skin tissue, HepG2 cells, pig colon tissue, mouse testis tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Sample-dependent, check data in validation data gallery |
Product Information
67275-1-Ig targets Biglycan in WB,ELISA applications and shows reactivity with Human, Pig, Mouse samples.
Tested Reactivity | Human, Pig, Mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Biglycan fusion protein Ag8012 |
Full Name | biglycan |
Calculated molecular weight | 42 kDa |
Observed molecular weight | 48 kDa |
GenBank accession number | BC004244 |
Gene symbol | BGN |
Gene ID (NCBI) | 633 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).
Protocols
Product Specific Protocols | |
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WB protocol for Biglycan antibody 67275-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |