C11orf68 Polyclonal antibody

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  WB analysis using 31587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31587-1-AP (C11orf68 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 31587-1-AP

  WB result of C11orf68 antibody (31587-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C11orf68 transfected A431 cells.

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  IHC staining of human stomach cancer using 31587-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31587-1-AP (C11orf68 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

C11orf68 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31587-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

BLES03, P5326, Protein p5326, UPF0696 protein C11orf68

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Tested Applications

• Positive WB detected in: rat pancreas tissue, A431 cells, mouse liver tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31587-1-AP targets C11orf68 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36023

  Product name: Recombinant human C11orf68 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-251 aa of BC010512

  Sequence: MEPGEELEEEGSPGGREDGFTAEHLAAEAMAADMDPWLVFDARTTPATELDAWLAKYPPSQVTRYGDPGSPNSEPVGWIAVYGQGYSPNSGDVQGLQAAWEALQTSGRPITPGTLRQLAITHHVLSGKWLMHLAPGFKLDHAWAGIARAVVEGRLQVAKVSPRAKEGGRQVICVYTDDFTDRLGVLEADSAIRAAGIKCLLTYKPDVYTYLGIYRANRWHLCPTLYESRFQLGGSARGSRVLDRANNVELT

• Full Name: chromosome 11 open reading frame 68
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC010512
• Gene Symbol: C11orf68
• Gene ID (NCBI): 83638
• RRID: AB_3670046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H3H3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BLES03 (basophilic leukemia-expressed protein 03) is a human protein of unknown function. Recent studies have shown that BLES03 is a novel eukaryotic phosphothreonine cleavage enzyme involved in the biosynthesis of dehydrogenated amino acids.The crystal structure of BLES03 has been resolved by single-wavelength anomalous scattering (SAD) with a resolution of 2.5 Å.