IFT43 Polyclonal antibody

IFT43 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 24338-1-AP

Synonyms

C14orf179, IFT43



Tested Applications

Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24338-1-AP is IFT43 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT43 fusion protein Ag19434
Full Name chromosome 14 open reading frame 179
Calculated molecular weight 213 aa, 24 kDa
GenBank accession numberBC010436
Gene symbol C14orf179
Gene ID (NCBI) 112752
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFT43, encoded by C14ORF179, is a subunit of the intraflagellar transport complex A (IFT-A) of primary cilia. The IFT-A is implicated in retrograde ciliary transport along axonemal microtubules. Defects in subunits of IFT-A has been associated with skeletal ciliopathies, including Sensenbrenner syndrome, Jeune syndrome, and Ellis-van-Creveld syndrome. Mutations in C14ORF179 has been linked to Sensenbrenner syndrome. (21378380)

Protocols

Product Specific Protocols
IHC protocol for IFT43 antibody 24338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Physiol

Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.

Authors - Caleb S Hayes
mouse

Hum Mol Genet

Ift172 conditional knock-out mice exhibit rapid retinal degeneration and protein trafficking defects.

Authors - Priya R Gupta
humanIF

Elife

Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function.

Authors - Laura Vuolo
mouseIF

Proc Natl Acad Sci U S A

Primary cilia signaling mediates intraocular pressure sensation.

Authors - Na Luo
mouseWB

Brain

De novo purine biosynthesis is a major driver of chemoresistance in glioblastoma.

Authors - Jack M Shireman