|Positive IHC detected in
|human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|MDCK cells, hTERT-RPE1 cells
|IHC : 1:20-1:200
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
24338-1-AP targets IFT43 in WB, IHC, IF, ELISA applications and shows reactivity with human, canine samples.
|Host / Isotype
|Rabbit / IgG
|IFT43 fusion protein Ag19434
|chromosome 14 open reading frame 179
|Calculated molecular weight
|213 aa, 24 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
IFT43, encoded by C14ORF179, is a subunit of the intraflagellar transport complex A (IFT-A) of primary cilia. The IFT-A is implicated in retrograde ciliary transport along axonemal microtubules. Defects in subunits of IFT-A has been associated with skeletal ciliopathies, including Sensenbrenner syndrome, Jeune syndrome, and Ellis-van-Creveld syndrome. Mutations in C14ORF179 has been linked to Sensenbrenner syndrome. (21378380)
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Ift172 conditional knock-out mice exhibit rapid retinal degeneration and protein trafficking defects.
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