Tested Applications
| Positive IF-P detected in | mouse pancreas tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells, MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-17711 targets ARL13B in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12015 Product name: Recombinant human ARL13B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-321 aa of BC094725 Sequence: MFSLMASCCGWFKRWREPVRLANKQDKEGALGEADVIECLSLEKLVNEHKCLCQIEPCSAISGYGKKIDKSIKKGLYWLLHVIARDFDALNERIQKETTEQRALEEQEKQERAERVRKLREERKQNEQEQAELDGTSGLAELDPEPTNPFQPIASVIIENEGKLEREKKNQKMEKDSDGCHLKHKMEHEQIETQGQVNHNGQKNNEFGLVENYKEALTQQLKNEDETDRPSLESANGKKKTKKLRMKRNHRVEPLNIDDCAPESPTPPPPPPPVGWGTPKVTRLPKLEPLGETHHNDFYRKPLPPLAVPQRPNSDAHDVIS Predict reactive species |
| Full Name | ADP-ribosylation factor-like 13B |
| Calculated Molecular Weight | 48 kDa |
| GenBank Accession Number | BC094725 |
| Gene Symbol | ARL13B |
| Gene ID (NCBI) | 200894 |
| RRID | AB_2883085 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3SXY8 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). CoraLite488-Conjugated ARL13B antibody can be used to mark the cilia(green). Ex: 488nm, Em: 515nm
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ARL13B antibody CL488-17711 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL488-17711 is the CoraLite® Plus 488-conjugated ARL13B Polyclonal antibody. It has 3 citations published in Molecular Oncology, Neuroscience Research, and medRxiv. The associated research fields include epigenetic regulation in melanoma, cilia biology in CDKL5 Deficiency Disorder, and antisense oligonucleotide therapy for ALS. All three studies applied the antibody for immunofluorescence (IF).
| Species | Application | Title |
|---|---|---|
Mol Oncol Interferon regulatory factor 4 modulates epigenetic silencing and cancer-critical pathways in melanoma cells | ||
Neurosci Res Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder. | ||
medRxiv Antisense oligonucleotide depletion of CCDC146 is a broad-spectrum therapeutic strategy for ALS |
Reviews
What customers say
The single review for CL488-17711 (lot 21003417) is a 5-star rating from October 2025. The reviewer, Lola Albertella, used the antibody at 1:500 dilution for immunofluorescence on primary myoblasts. She reported that the conjugated antibody performs well with very low background noise, making it ideal for cilia imaging and suitable for expansion microscopy applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lola (Verified Customer) (10-23-2025) | This conjugated antibody works well, with very low background noise. Perfect for cilia imaging. Suitable for expansion microscopy.
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