Various lysates were subjected to SDS PAGE followed by western blot with 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IHC staining of human liver cancer using 68131-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 68131-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 68131-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYDGF/C19orf10 antibody (68131-1-Ig, Clone: 1H8F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYDGF/C19orf10 antibody (68131-1-Ig, Clone: 1H8F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
FC experiment of HepG2 using 68131-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MYDGF/C19orf10 (68131-1-Ig, Clone:1H8F4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MYDGF/C19orf10 (68131-1-Ig, Clone:1H8F4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
68131-1-Ig targets MYDGF/C19orf10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769).
Protocols
Product Specific Protocols
WB protocol for MYDGF/C19orf10 antibody 68131-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IHC Figures
IHC staining of human liver cancer using 68131-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 68131-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 68131-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYDGF/C19orf10 antibody (68131-1-Ig, Clone: 1H8F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of HepG2 using 68131-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MYDGF/C19orf10 (68131-1-Ig, Clone:1H8F4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
MYDGF/C19orf10 Monoclonal antibody
Catalog Number
68131-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF/ICC : 1:400-1:1600 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
