C1QA Recombinant antibody

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  WB analysis using 85719-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85719-4-RR (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85719-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1QA antibody (85719-4-RR, Clone: 250093H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C1QA Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85719-4-RR

Clone No.250093H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Complement C1q A chain

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Tested Applications

• Positive WB detected in: mouse spleen tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85719-4-RR targets C1QA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: C1QA fusion protein Eg2396
• Full Name: complement component 1, q subcomponent, A chain
• Calculated Molecular Weight: 26kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: NM_015991.4
• Gene Symbol: C1QA
• Gene ID (NCBI): 712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02745
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.