C1S Recombinant monoclonal antibody

• 

  WB analysis of A431 using 85736-6-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 85736-6-RR (C1S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85736-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85736-6-RR (C1S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 85736-6-RR

  Biolayer interferometry (BLl) kinetic assays of 85736-6-RR against Human C1S were performed. The affinity constant is 1.63 nM.

C1S Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85736-6-RR

Clone No.250031G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Complement C1s, C1 esterase, Complement C1s subcomponent, EC:3.4.21.42

---

Tested Applications

• Positive WB detected in: A431 cells, human plasma, human serum

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85736-6-RR targets C1S in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5977

  Product name: Recombinant human C1S protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 166-688 aa of BC056903

  Sequence: DDMKNCGVNCSGDVFTALIGEIASPNYPKPYPENSRCEYQIRLEKGFQVVVTLRREDFDVEAADSAGNCLDSLVFVAGDRQFGPYCGHGFPGPLNIETKSNALDIIFQTDLTGQKKGWKLRYHGDPMPCPKEDTPNSVWEPAKAKYVFRDVVQITCLDGFEVVEGRVGATSFYSTCQSNGKWSNSKLKCQPVDCGIPESIENGKVEDPESTLFGSVIRYTCEEPYYYMENGGGGEYHCAGNGSWVNEVLGPELPKCVPVCGVPREPFEEKQRIIGGSDADIKNFPWQVFFDNPWAGGALINEYWVLTAAHVVEGNREPTMYVGSTSVQTSRLAKSKMLTPEHVFIHPGWKLLEVPEGRTNFDNDIALVRLKDPVKMGPTVSPICLPGTSSDYNLMDGDLGLISGWGRTEKRDRAVRLKAARLPVAPLRKCKEVKVEKPTADAEAYVFTPNMICAGGEKGMDSCKGDSGGAFAVQDPNDKTKFYAAGLVSWGPQCGTYGLYTRVKNYVDWIMKTMQENSTPRED

• Full Name: complement component 1, s subcomponent
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC056903
• Gene Symbol: C1S
• Gene ID (NCBI): 716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09871
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r activates C1s so that it can, in turn, activate C2 and C4. C1s is a glycoprotein of approximately 88 kDa, which is activated by cleavage into an A chain and a B chain.