|Positive WB detected in||PC-3 cells, L02 cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human tonsillitis tissue, human lung tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells, PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21316-1-AP is C5aR Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||C5aR fusion protein Ag15968|
|Full Name||complement component 5a receptor 1|
|Calculated molecular weight||350 aa, 39 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC008982|
|Gene ID (NCBI)||728|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
C5aR, also named as CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).
|Product Specific Protocols|
|WB protocol for C5aR antibody 21316-1-AP||Download protocol|
|IHC protocol for C5aR antibody 21316-1-AP||Download protocol|
|IF protocol for C5aR antibody 21316-1-AP||Download protocol|
|IP protocol for C5aR antibody 21316-1-AP||Download protocol|
|FC protocol for C5aR antibody 21316-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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