C5aR Polyclonal antibody

• Featured Product
• KD/KO Validated

C5aR Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

27

Reviews (1)

Cat no : 21316-1-AP

Synonyms

C5A, C5a R, C5aR, C5AR1, C5R1, CD88

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Validation Data Gallery

• 

  WB analysis using 21316-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21316-1-AP (C5aR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21316-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21316-1-AP (C5aR antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21316-1-AP (C5aR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21316-1-AP (C5aR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21316-1-AP (C5aR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21316-1-AP (C5aR antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21316-1-AP (C5aR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 21316-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21316-1-AP (C5aR antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21316-1-AP

  Immunofluorescent analysis of HepG2 cells, using C5AR1 antibody 21316-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 21316-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human C5aR (21316-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: L02 cells, PC-3 cells, U-937 cells
• Positive IHC detected in: human tonsillitis tissue, human lung tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 6 publications below
• IF: See 9 publications below
• FC: See 1 publications below

Product Information

21316-1-AP targets C5aR in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C5aR fusion protein Ag15968
• Full Name: complement component 5a receptor 1
• Calculated Molecular Weight: 350 aa, 39 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC008982
• Gene Symbol: C5AR1
• Gene ID (NCBI): 728
• RRID: AB_10733105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C5aR, also named as CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).

Publications

Species	Application	Title
	WB	Immunity; Complement is a central mediator of radiotherapy-induced tumor-specific immunity and clinical response.; Authors - Laura Surace
	WB	Theranostics; The hepatocyte-specifically expressed lnc-HSER alleviates hepatic fibrosis by inhibiting hepatocyte apoptosis and epithelial-mesenchymal transition.; Authors - Kun Zhang
human	IF	Sci Rep; Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth.; Authors - Fanny Chmilewsky
pig	WB	Sci Rep; Cardiac Depression in Pigs after Multiple Trauma - Characterization of Posttraumatic Structural and Functional Alterations.; Authors - M Kalbitz
human	WB	Cell Signal; The C5aR1 complement receptor: A novel immunomodulator of insulin action in skeletal muscle; Authors - Dinesh S Shah ; KO Validated
human	IF	Front Immunol; Complement Activation and Organ Damage After Trauma-Differential Immune Response Based on Surgical Treatment Strategy.; Authors - Ina Lackner

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Muhammad (Verified Customer) (12-25-2022)

one of the important receptor of complement system and this antibody helped a lot in our study to examine its role in regenerative medicine.

• Applications: Immunohistochemistry, Immunofluorescence
• Cell Tissue Type: MSC's/Fibroblasts/DPSC's

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