Tested Applications
| Positive WB detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25249-1-AP targets C6orf130 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19382 Product name: Recombinant human C6orf130 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-152 aa of BC011709 Sequence: MASSLNEDPEGSRITYVKGDLFACPKTDSLAHCISEDCRMGAGIAVLFKKKFGGVQELLNQQKKSGEVAVLKRDGRYIYYLITKKRASHKPTYENLQKSLEAMKSHCLKNGVTDLSMPRIGCGLDRLQWENVSAMIEEVFEATDIKITVYTL Predict reactive species |
| Full Name | chromosome 6 open reading frame 130 |
| Calculated Molecular Weight | 152 aa, 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC011709 |
| Gene Symbol | C6orf130 |
| Gene ID (NCBI) | 221443 |
| RRID | AB_2753118 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y530 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OARD1 (O-acetyl-ADP-ribose deacetylase 1), also known as TARG1 (Terminal ADP-ribose protein glycohydrolase 1) and C6orf130, is a ADP-ribose glycohydrolase that hydrolyzes ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on glutamate and O-acetyl-ADP-D-ribose (PMID: 21849506; 23474714; 23481255). The MW of this protein is 17 kDa, and this antibody specially recognises the 17 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C6orf130 antibody 25249-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The C6orf130 (TARG1/OARD1) Polyclonal antibody (Cat# 25249-1-AP) has 5 citations, all for human WB. They appear in Molecular Cell, Nucleic Acids Research, Cell Reports (two), and Scientific Reports. Research fields include PARP-mediated ADP-ribosylation, TARG1/PARG regulation of genomic stability, proteasomal degradation, and EGFR signaling via RNA metabolism.
| Species | Application | Title |
|---|---|---|
Cell Rep The interplay of TARG1 and PARG protects against genomic instability
| ||
Reviews
What customers say
The single review for this antibody rates it 4 out of 5. The reviewer used it for Western Blot at a 1:1000 dilution and described it as a generally good working antibody, though not outstanding. Overall, the feedback is positive with a minor note that performance is solid rather than exceptional.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lisa (Verified Customer) (09-17-2025) | Generally a good working antibody, but not outstanding
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