CACNA2D1 Polyclonal antibody

CACNA2D1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 27453-1-AP

Synonyms

CACNA2, CACNA2D1, CACNL2A, CCHL2A, MHS3

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Validation Data Gallery

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  WB analysis of mouse brain using 27453-1-AP

  mouse brain tissue and mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 27453-1-AP (CACNA2D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 27453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 27453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, pig brain tissue, rat brain tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

27453-1-AP targets CACNA2D1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CACNA2D1 fusion protein Ag26190
• Full Name: calcium channel, voltage-dependent, alpha 2/delta subunit 1
• Calculated molecular weight: 125 kDa
• Observed molecular weight: 125 kDa
• GenBank accession number: NM_000722
• Gene symbol: CACNA2D1
• Gene ID (NCBI): 781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christian (Verified Customer) (11-19-2018)

1:50 - labelled neurons of interest1:200 - labelled 3-4 neurons of interest1:1000 - labelled 1-2 neurons of interest

• Applications: Immunohistochemistry,Immunofluorescence,
• Primary Antibody Dilution: 1:50, 1:200, 1:1000
• Cell Tissue Type: Mouse Spinal Cord/Neurons

CACNA2D1 Antibody Immunohistochemistry,Immunofluorescence, validation (1:50, 1:200, 1:1000 dilution) in Mouse Spinal Cord/Neurons (Cat no:27453-1-AP)