Validation Data Gallery
|Positive WB detected in
|mouse brain tissue, pig brain tissue, rat brain tissue, mouse skeletal muscle tissue
|Positive IHC detected in
|human skeletal muscle tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|mouse eye tissue
|Western Blot (WB)
|WB : 1:1000-1:4000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
27453-1-AP targets CACNA2D1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
|human, mouse, pig, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|CACNA2D1 fusion protein Ag26190
|calcium channel, voltage-dependent, alpha 2/delta subunit 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Brain Behav Immun
Microglial IL-10 and β-endorphin expression mediates gabapentinoids antineuropathic pain.
Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1
Calcium-Related Genes Predicting Outcomes and Serving as Therapeutic Targets in Endometrial Cancer
Ecotoxicol Environ Saf
Cigarette smoke triggers calcium overload in mouse hippocampal neurons via the ΔFOSB-CACNA2D1 axis to impair cognitive performance
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christian (Verified Customer) (11-19-2018)
1:50 - labelled neurons of interest1:200 - labelled 3-4 neurons of interest1:1000 - labelled 1-2 neurons of interest