CACNA2D1 Polyclonal antibody

CACNA2D1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27453-1-AP

Synonyms

CACNA2, CACNA2D1, CACNL2A, CCHL2A, MHS3



Tested Applications

Positive WB detected inmouse brain tissue, pig brain tissue, rat brain tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

27453-1-AP targets CACNA2D1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CACNA2D1 fusion protein Ag26190
Full Name calcium channel, voltage-dependent, alpha 2/delta subunit 1
Calculated molecular weight 125 kDa
Observed molecular weight 125 kDa
GenBank accession numberNM_000722
Gene symbol CACNA2D1
Gene ID (NCBI) 781
RRIDAB_2880874
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CACNA2D1 antibody 27453-1-APDownload protocol
IHC protocol for CACNA2D1 antibody 27453-1-APDownload protocol
IF protocol for CACNA2D1 antibody 27453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF,WB

Brain Behav Immun

Microglial IL-10 and β-endorphin expression mediates gabapentinoids antineuropathic pain.

Authors - Khalil Ali Ahmad
ratWB,IF

Heliyon

Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1

Authors - Chuan Jiang
humanWB,IF

Cells

Calcium-Related Genes Predicting Outcomes and Serving as Therapeutic Targets in Endometrial Cancer

Authors - Ting Huang
mouseWB,IF

Ecotoxicol Environ Saf

Cigarette smoke triggers calcium overload in mouse hippocampal neurons via the ΔFOSB-CACNA2D1 axis to impair cognitive performance

Authors - Hongchao Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christian (Verified Customer) (11-19-2018)

1:50 - labelled neurons of interest1:200 - labelled 3-4 neurons of interest1:1000 - labelled 1-2 neurons of interest

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:50, 1:200, 1:1000
  • Cell Tissue Type: Mouse Spinal Cord/Neurons
CACNA2D1 Antibody Immunohistochemistry,Immunofluorescence, validation (1:50, 1:200, 1:1000 dilution) in Mouse Spinal Cord/Neurons (Cat no:27453-1-AP)