CoraLite® Plus 488-conjugated CAMK2 Monoclonal antibody

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  WB analysis using CL488-66843

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66843 (CAMK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CAMK2 Monoclonal Antibody for WB

Cat No. CL488-66843

Clone No.1E3B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB

Synonyms

CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMKA, CaMKII alpha, CaMK-II subunit alpha

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66843 targets CAMK2 in WB applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4736

  Product name: Recombinant human CAMK2A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-271 aa of BC040457

  Sequence: MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWI

• Full Name: calcium/calmodulin-dependent protein kinase II alpha
• Calculated Molecular Weight: 478 aa, 54 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: BC040457
• Gene Symbol: CaMKII alpha
• Gene ID (NCBI): 815
• RRID: AB_3084277
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UQM7
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It has 10 potential isoforms with a molecular mass range from 21 to 63 kDa.