CCL11/Eotaxin Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA sample test of MP03000-1

  Plasma of six individual healthy human donors was measured. The CCL11/Eotaxin concentration of detected samples was determined to be 284.4 pg/mL with a range of 95.7-531.2 pg/mL.

• 

  Sandwich ELISA sample test of MP03000-1

  Serum of eight individual healthy human donors was measured. The CCL11/Eotaxin concentration of detected samples was determined to be 206.0 pg/mL with a range of 70.9-418.0 pg/mL.

• 

  Sandwich ELISA standard curve of MP03000-1

  Sandwich ELISA standard curve of MP03000-1, Human CCL11/Eotaxin Recombinant Matched Antibody Pair - PBS only. 87475-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg5095. 87475-1-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL.

• 

  Affinity and Kinetic Characterization of 87475-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 87475-1-PBS against Human CCL11/Eotaxin was performed. The affinity constant is 5.44 nM.

CCL11/Eotaxin Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 87475-1-PBS

Clone No.251987D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

CCL11, Eotaxin, C C motif chemokine 11, C-C motif chemokine 11, CCL 11

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Product Information

87475-1-PBS targets CCL11/Eotaxin as part of a matched antibody pair:

MP03000-1: 87475-2-PBS capture and 87475-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5095

  Product name: recombinant human Eotaxin protein

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 24-97 aa of BC017850

  Sequence: GPASVPTTCCFNLANRKIPLQRLESYRRITSGKCPQKAVIFKTKLAKDICADPKKKWVQDSMKYLDQKSPTPKP

• Full Name: chemokine (C-C motif) ligand 11
• Calculated Molecular Weight: 97 aa, 11 kDa
• GenBank Accession Number: BC017850
• Gene Symbol: CCL11/Eotaxin
• Gene ID (NCBI): 6356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P51671
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.