cyclin I Polyclonal antibody

cyclin I Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16357-1-AP

Synonyms

CCNI, CYC1, cyclin I, CYI

---

Validation Data Gallery

• 

  WB analysis using 16357-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16357-1-AP (cyclin I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16357-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16357-1-AP (cyclin I antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 16357-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16357-1-AP (cyclin I antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16357-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat brain tissue
• Positive IHC detected in: human heart tissue, human brain tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16357-1-AP targets cyclin I in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: cyclin I fusion protein Ag0493
• Full Name: cyclin I
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC004975
• Gene Symbol: CCNI
• Gene ID (NCBI): 10983
• RRID: AB_2275606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.