CCT3 Monoclonal antibody

CCT3 Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

5F5A6

Cat no : 60264-1-Ig

Synonyms

CCT gamma, CCT3, CCTG, hTRiC5, PIG48, TCP 1 gamma, TRIC5

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Validation Data Gallery

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  WB analysis of COLO 320 using 60264-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60264-1-Ig (CCT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 60264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 60264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 60264-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

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  IF Staining of HepG2 using 60264-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (60264-1-Ig, Clone: 5F5A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 60264-1-Ig

  1X10^6 HepG2 cells were stained with 0.2ug CCT3 antibody (60264-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: COLO 320 cells
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

60264-1-Ig targets CCT3 in WB, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCT3 fusion protein Ag0843
• Full Name: chaperonin containing TCP1, subunit 3 (gamma)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC006501
• Gene Symbol: CCT3
• Gene ID (NCBI): 7203
• RRID: AB_2881385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IF,CoIP	Front Cell Dev Biol; Usher syndrome proteins ADGRV1 (USH2C) and CIB2 (USH1J) interact and share a common interactome containing TRiC/CCT-BBS chaperonins; Authors - Joshua Linnert