CoraLite®594-conjugated CCT7 Monoclonal antibody

CCT7 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2C1G1

Cat no : CL594-67540

Synonyms

CCT ETA, CCT7, Ccth, HIV 1 Nef interacting protein, Nip7 1, TCP 1 eta

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Validation Data Gallery

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  IF Staining of U2OS using CL594-67540

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite®594 CCT7 antibody (CL594-67540, Clone: 2C1G1 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67540 targets CCT7 in IF applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCT7 fusion protein Ag9502
• Full Name: chaperonin containing TCP1, subunit 7 (eta)
• Calculated Molecular Weight: 543 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC019296
• Gene Symbol: CCT7
• Gene ID (NCBI): 10574
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.