CD107a / LAMP1 Polyclonal antibody

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  WB analysis using 32435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32435-1-AP (CD107a / LAMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 32435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32435-1-AP (CD107a / LAMP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 32435-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Lamp1 antibody (32435-1-AP) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

CD107a / LAMP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 32435-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

CD107a, Lamp1, Lamp 1

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, C6 cells, HSC-T6 cells, rat kidney tissue, RAW 264.7 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32435-1-AP targets CD107a / LAMP1 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD107a / LAMP1 fusion protein Ag34623
• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 90-120 kDa
• GenBank Accession Number: NM_001317353
• Gene Symbol: CD107a
• Gene ID (NCBI): 16783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P11438
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.