Mouse CD11a Recombinant monoclonal antibody, PBS Only

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• Mouse CD11a Recombinant monoclonal antibody, PBS Only (87542-1-PBS)

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  WB analysis using 87542-1-RR (same clone as 87542-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87542-1-RR (ITAL antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87542-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 87542-1-RR (same clone as 87542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87542-1-RR (CD11a antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87542-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 87542-1-RR (same clone as 87542-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed RAW 264.7 cells using CD11a antibody (87542-1-RR, Clone: 252754D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87542-1-PBS in a different storage buffer formulation.

CD11a Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 87542-1-PBS

Clone No.252754D9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Itgal, integrin alpha L, LFA 1

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Product Information

87542-1-PBS targets CD11a in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10464

  Product name: Recombinant mouse ITAL protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011451 of

  Sequence: \

• Full Name: integrin alpha L
• Observed Molecular Weight: 180 kDa
• Gene Symbol: CD11a
• Gene ID (NCBI): 16408
• RRID: AB_3745611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: B7ZN92
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).