CD11a Recombinant monoclonal antibody

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• CD11a Recombinant monoclonal antibody (87542-1-RR)

• 

  WB analysis using 87542-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87542-1-RR (ITAL antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 87542-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87542-1-RR (CD11a antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 87542-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed RAW 264.7 cells using CD11a antibody (87542-1-RR, Clone: 252754D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD11a Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 87542-1-RR

Clone No.252754D9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Itgal, integrin alpha L, LFA 1

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Tested Applications

• Positive WB detected in: mouse spleen tissue, mouse thymus tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87542-1-RR targets CD11a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10464

  Product name: Recombinant mouse ITAL protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011451 of

  Sequence: \

• Full Name: integrin alpha L
• Observed Molecular Weight: 180 kDa
• Gene Symbol: CD11a
• Gene ID (NCBI): 16408
• RRID: AB_3745611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: B7ZN92
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).