Anti-Human CD134/OX40 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98181-1-RR

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD134/OX40 Rabbit Recombinant Antibody (98181-1-RR, Clone: 241398G7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD4 (RPA-T4) (APC-65143, Clone: RPA-T4). Cells were not fixed.

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  FC experiment of human PBMCs using 98181-1-RR

  1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD134/OX40 Rabbit Recombinant Antibody (98181-1-RR, Clone: 241398G7) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD134/OX40 Rabbit Recombinant Antibody (98181-1-RR, Clone: 241398G7) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98181-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98181-1-RR against Human CD134/OX40 were performed. The affinity constant is 5.48 nM.

CD134/OX40 Recombinant Antibody for FC

Cat No. 98181-1-RR

Clone No.241398G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TNFRSF4, OX40L receptor, OX40, CD134, ACT35 antigen

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98181-1-RR targets CD134/OX40 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: tumor necrosis factor receptor superfamily, member 4
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: NM_003327
• Gene Symbol: CD134
• Gene ID (NCBI): 7293
• RRID: AB_3672322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P43489
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).