CoraLite®594 Anti-Human CD134/OX40 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL594-98181

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD4 and CoraLite®594 Anti-Human CD134/OX40 Rabbit RecAb (CL594-98181, Clone: 241398G7). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL594-98181

  1x10^6 PHA-treated human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD134/OX40 Rabbit RecAb (CL594-98181, Clone: 241398G7) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD134/OX40 Rabbit RecAb (CL594-98181, Clone: 241398G7) (black, dashed). Cells were not fixed.

CD134/OX40 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL594-98181

Clone No.241398G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TNFRSF4, 241398G7, ACT35, ACT35 antigen, CD134

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Tested Applications

• Positive FC detected in: PHA-treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98181 targets CD134/OX40 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD134/OX40 fusion protein Eg1261
• Full Name: tumor necrosis factor receptor superfamily, member 4
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: NM_003327
• Gene Symbol: CD134
• Gene ID (NCBI): 7293
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43489
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).