PE Anti-Human CD146 (P1H12)

• 

  FC experiment of A375 using PE-65181

  1X10^6 A375 cells were surface stained with 5 ul PE Anti-Human CD146 (PE-65181, Clone: P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

• 

  FC experiment of A375 using PE-65181

  1X10^6 A375 cells were surface stained with 5 ul PE Anti-Human CD146 (PE-65181, Clone: P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

CD146 Monoclonal Antibody for FC

Cat No. PE-65181

Clone No.P1H12

Publications(2)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, mouse, rabbit, canine

Applications

FC

Synonyms

S-endo 1 endothelial-associated antigen, P1H12, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32

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Tested Applications

• Positive FC detected in: A375 cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

PE-65181 targets CD146 in FC applications and shows reactivity with human, mouse, rabbit, canine samples.

• Tested Reactivity: human, mouse, rabbit, canine
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: cultured human umbilical cells
• Full Name: melanoma cell adhesion molecule
• Calculated Molecular Weight: 646 aa, 72 kDa
• GenBank Accession Number: BC056418
• Gene Symbol: CD146
• Gene ID (NCBI): 4162
• RRID: AB_2883952
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• UNIPROT ID: P43121
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Publications

Species	Application	Title
human	FC	J Dent Sci; N-acetylcysteine promotes cyclic mechanical stress-induced osteogenic differentiation of periodontal ligament stem cells by down-regulating Nrf2 expression.; Authors - Xun Xi
human	FC	J Tissue Eng; Enhanced effects of antagomiR-3074-3p-conjugated PEI-AuNPs on the odontogenic differentiation by targeting FKBP9; Authors - Tao Jiang