PE Anti-Human CD146 (P1H12)

CD146 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1

Reactivity

Rabbit, Canine, Mouse, Human and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

1

CloneNo.

P1H12

Cat no : PE-65181

Synonyms

CD146, MCAM, MUC18

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Validation Data Gallery

• 

  FC experiment of HeLa using PE-65181

  1X10^6 HeLa cells were surface stained with 10.00 ul/test PE Anti-Human CD146 (PE-65181, Clone:P1H12) (red) or isotype control antibody (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Published Applications

• FC: See 1 publications below

Product Information

PE-65181 targets CD146 in FC applications and shows reactivity with Rabbit, Canine, Mouse, Human samples.

• Tested Reactivity: Rabbit, Canine, Mouse, Human
• Cited Reactivity: human
• Host / Isotype: Mouse / Mouse IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: cultured human umbilical cells
• Full Name: melanoma cell adhesion molecule
• Calculated molecular weight: 646 aa, 72 kDa
• GenBank accession number: BC056418
• Gene symbol: MCAM
• Gene ID (NCBI): 4162
• Conjugate: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	FC	J Dent Sci; N-acetylcysteine promotes cyclic mechanical stress-induced osteogenic differentiation of periodontal ligament stem cells by down-regulating Nrf2 expression.; Authors - Xun Xi