CD151 Polyclonal antibody

CD151 Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10418-1-AP

Synonyms

CD151, CD151 antigen, GP27, Membrane glycoprotein SFA 1, MER2, PETA 3, RAPH, SFA1, Tetraspanin 24, Tspan 24, TSPAN24

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Validation Data Gallery

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  WB analysis of mouse spleen using 10418-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10418-1-AP (CD151 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 10418-1-AP

  IP Result of anti-CD151 (IP:10418-1-AP, 3ug; Detection:10418-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

• 

  FC experiment of A549 using 10418-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human CD151 (10418-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse spleen tissue
• Positive IP detected in: mouse spleen tissue
• Positive FC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

10418-1-AP targets CD151 in WB, IP, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD151 fusion protein Ag0341
• Full Name: CD151 molecule (Raph blood group)
• Calculated molecular weight: 28 kDa
• Observed molecular weight: 32 kDa
• GenBank accession number: BC001374
• Gene symbol: CD151
• Gene ID (NCBI): 977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.

Publications

Species	Application	Title
rat	WB	FASEB J; Endothelial microparticles induced by cyclic stretch activate Src and modulate cell apoptosis.; Authors - Fei Zhuang
		Int J Mol Med; Tetraspanin CD9 is involved in the migration of retinal microvascular endothelial cells.; Authors - Deissler Heidrun H

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