CD151 Monoclonal antibody

CD151 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2A8G8

Cat no : 66567-1-Ig

Synonyms

CD151, CD151 antigen, GP27, Membrane glycoprotein SFA 1, MER2, PETA 3, RAPH, SFA1, Tetraspanin 24, Tspan 24, TSPAN24



Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman tonsillitis tissue,
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66567-1-Ig targets CD151 in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD151 fusion protein Ag25985
Full Name CD151 molecule (Raph blood group)
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC001374
Gene symbol CD151
Gene ID (NCBI) 977
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.

Protocols

Product Specific Protocols
WB protocol for CD151 antibody 66567-1-IgDownload protocol
IHC protocol for CD151 antibody 66567-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Free Radic Biol Med

Downregulation of CD151 induces oxidative stress and apoptosis in trophoblast cells via inhibiting ERK/Nrf2 signaling pathway in preeclampsia.

Authors - Zhiyin Wang
  • KD Validated