Anti-Human CD16b Rabbit Recombinant Antibody

• 

  FC experiment of human peripheral blood leukocytes using 98192-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98192-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98192-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98192-1-RR against Human CD16b were performed. The affinity constant is 2.36 nM.

CD16b Uni-rAb^® Recombinant Antibody for FC

Cat No. 98192-1-RR

Clone No.241713E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD16, FCGR3B, 241713E3, Fc gamma RIII, Fc gamma RIII beta

---

Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98192-1-RR targets CD16b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2487

  Product name: recombinant human CD16b protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007018 of

  Sequence:

• Full Name: Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
• Gene Symbol: FCGR3B
• Gene ID (NCBI): 2215
• RRID: AB_3672329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins (PMID: 30361439).