PE Anti-Human CD16b Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using PE-98192

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human CD16b Rabbit RecAb (PE-98192, Clone: 241713E3) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using PE-98192

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human CD16b Rabbit RecAb (PE-98192, Clone: 241713E3) (red) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD16b Uni-rAb^® Recombinant Antibody for FC

Cat No. PE-98192

Clone No.241713E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD16, FCGR3B, 241713E3, Fc gamma RIII, Fc gamma RIII beta

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-98192 targets CD16b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2487

  Product name: recombinant human CD16b protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007018 of

  Sequence:

• Full Name: Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
• Gene Symbol: FCGR3B
• Gene ID (NCBI): 2215
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Excitation Laser: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins (PMID: 30361439).