FITC Plus Anti-Human CD1a (HI149)

CD1a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

HI149

Cat no : FITC-65172

Synonyms

hTa1 thymocyte antigen, HTA1, HI149, FCB6, CD1a molecule

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Validation Data Gallery

• 

  FC experiment of MOLT-4 using FITC-65172

  1X10^6 MOLT-4 cells were surface stained with 5 ul FITC Plus Anti-Human CD1a (FITC-65172, Clone:HI149) or Mouse IgG1 Isotype Control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: MOLT-4 cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65172 targets CD1a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human thymocytes
• Full Name: CD1a molecule
• Calculated Molecular Weight: 327 aa, 37 kDa
• GenBank Accession Number: BC031645
• Gene Symbol: CD1A
• Gene ID (NCBI): 909
• RRID: AB_2883813
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface.